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Western Blot analysis of Human serum using Transferrin Monoclonal Antibody at dilution of 1:2000.
Western Blot analysis of Human serum using Transferrin Monoclonal Antibody at dilution of 1:2000.
Western Blot analysis of Human serum using Transferrin Monoclonal Antibody at dilution of 1:2000.

Transferrin Monoclonal Antibody

E-AB-22178
Elabscience
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTF
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Overview

  • Supplier
    Elabscience
  • Product Name
    Transferrin Monoclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5A4
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7018
  • Target name
    TF
  • Target description
    transferrin
  • Target synonyms
    HEL-S-71p, PRO1557, PRO2086, TFQTL1, serotransferrin, beta-1 metal-binding globulin, epididymis secretory sperm binding protein Li 71p, siderophilin
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDP02787
  • Protein Name
    Serotransferrin
  • Scientific Description
    This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of Transferrin using anti-Transferrin antibody (PB9827). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse Hepa1/6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Transferrin antigen affinity purified polyclonal antibody (Catalog # PB9827) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Transferrin at approximately 77 kDa. The expected band size for Transferrin is at 77 kDa.
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