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Trident Membrane Protein Extraction Kit

Research Use Only
GTX16373
GeneTex
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Product group Molecular Biology
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Overview

  • Supplier
    GeneTex
  • Product Name
    Trident Membrane Protein Extraction Kit
  • Delivery Days Customer
    9
  • Application Supplier Note
    1. Read the entire protocol carefully. 2. Before experiment, thaw buffer A or/and buffer B completely, invert the bottles a few times and keep them on ice. Place the filter cartridges in collection tubs, and pre-chill on ice. Pre-chill PBS on ice. 3. For IP, dissolve the extracted plasma membrane in PBS containing 0.5% Triton-X 100. Then do IP assay.
  • Certification
    Research Use Only
  • Scientific Description
    Trident Membrane protein extraction kit is for rapid extraction of native total membrane proteins from cultured mammalian cells or tissues. Simple and user friendly Wide range of starting cells (1 - 50 millions / sample) Detergent and EDTA free No need for Dounce homogenizer or tissue blender The procedure can be completed in less than 45 min. High yield
  • Storage Instruction
    -20°C,RT
  • UNSPSC
    41106510

References

  • A novel chimeric antigen receptor (CAR) system using an exogenous protease, in which activation of T cells is controlled by expression patterns of cell-surface proteins on target cells. Aoyama S et al., 2022 Apr, Int J Mol Med
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  • ANKRD22 is an N-myristoylated hairpin-like monotopic membrane protein specifically localized to lipid droplets. Utsumi T et al., 2021 Sep 28, Sci Rep
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  • The ESCRT-III molecules regulate the apical targeting of bile salt export pump. Wu SH et al., 2021 Mar 9, J Biomed Sci
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  • Identification and characterization of protein N-myristoylation occurring on four human mitochondrial proteins, SAMM50, TOMM40, MIC19, and MIC25. Utsumi T et al., 2018, PLoS One
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  • Intracellular cholesterol level regulates sensitivity of glioblastoma cells against temozolomide-induced cell death by modulation of caspase-8 activation via death receptor 5-accumulation and activation in the plasma membrane lipid raft. Yamamoto Y et al., 2018 Jan 1, Biochem Biophys Res Commun
    Read more