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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA868285LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA868285LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA868285LA01HU at dilution of 1:100

TRIM36 Antibody

CSB-PA868285LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRIM36
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Overview

  • Supplier
    Cusabio
  • Product Name
    TRIM36 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55521
  • Target name
    TRIM36
  • Target description
    tripartite motif containing 36
  • Target synonyms
    ANPH; ANPH1; E3 ubiquitin-protein ligase TRIM36; HAPRIN; RBCC728; RING finger protein 98; RING-type E3 ubiquitin transferase TRIM36; RNF98; tripartite motif protein 36; tripartite motif-containing protein 36; zinc-binding protein Rbcc728
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NQ86
  • Protein Name
    E3 ubiquitin-protein ligase TRIM36
  • Scientific Description
    E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Involved in chromosome segregation and cell cycle regulation. May play a role in the acrosome reaction and fertilization.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRIM36 using anti-TRIM36 antibody (A10270-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM36 antigen affinity purified polyclonal antibody (Catalog # A10270-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM36 at approximately 83 kDa. The expected band size for TRIM36 is at 83 kDa.
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