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TrkB antibody detects TrkB protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TrkB stained by TrkB antibody (GTX133722) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
TrkB antibody detects TrkB protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TrkB stained by TrkB antibody (GTX133722) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
TrkB antibody detects TrkB protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TrkB stained by TrkB antibody (GTX133722) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

TrkB antibody

GTX133722
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse, Rat
TargetNtrk2
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Overview

  • Supplier
    GeneTex
  • Product Name
    TrkB antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.24 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID18212
  • Target name
    Ntrk2
  • Target description
    neurotrophic tyrosine kinase, receptor, type 2
  • Target synonyms
    GP145-TrkB/GP95-TrkB, Tkrb, trk-B, trkB, BDNF/NT-3 growth factors receptor, neurotrophic tyrosine receptor kinase type 2, trkB tyrosine kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15209
  • Protein Name
    BDNF/NT-3 growth factors receptor
  • Scientific Description
    Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TrkB using anti-TrkB antibody (M01388-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TrkB antigen affinity purified monoclonal antibody (Catalog # M01388-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TrkB at approximately 92,140 kDa. The expected band size for TrkB is at 92 kDa.
Product group Antibodies
References
Boster Bio
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