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ELISA detection of TSG101 using GTX118736 for capture at a concentration of 5 microg/mL and GTX70255 for detection at a concentration of 1.5 microg/mL. The HRP-conjugated anti-mouse IgG antibody (GTX26789) was diluted at1:6,000-1:30,000 and used to detect the primary antibody.
ELISA detection of TSG101 using GTX118736 for capture at a concentration of 5 microg/mL and GTX70255 for detection at a concentration of 1.5 microg/mL. The HRP-conjugated anti-mouse IgG antibody (GTX26789) was diluted at1:6,000-1:30,000 and used to detect the primary antibody.
ELISA detection of TSG101 using GTX118736 for capture at a concentration of 5 microg/mL and GTX70255 for detection at a concentration of 1.5 microg/mL. The HRP-conjugated anti-mouse IgG antibody (GTX26789) was diluted at1:6,000-1:30,000 and used to detect the primary antibody.

TSG101 ELISA Pair

GTX500018
GeneTex
ApplicationsELISA
Product group Antibodies
TargetTSG101
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Overview

  • Supplier
    GeneTex
  • Product Name
    TSG101 ELISA Pair - TSG101 antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 5microg/mL and the detection antibody at a concentration of 1.5microg/mL. Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Gene ID7251
  • Target name
    TSG101
  • Target description
    tumor susceptibility 101
  • Target synonyms
    ESCRT-I complex subunit TSG101; TSG10; tumor susceptibility gene 10; tumor susceptibility gene 101; tumor susceptibility gene 101 protein; tumor susceptibility protein; VPS23
  • Scientific Description
    The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TSG101 using anti-TSG101 antibody (A01233-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Colo320 whole cell lysates, Lane 3: human Sw620 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human Hek293 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSG101 antigen affinity purified polyclonal antibody (Catalog # A01233-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSG101 at approximately 44KD. The expected band size for TSG101 is at 44KD.
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