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TSLP Mouse Monoclonal Antibody [Clone ID: 210H1]

TSLP Mouse Monoclonal Antibody [Clone ID: 210H1]

DDX0804P-100
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetTSLP
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    TSLP Mouse Monoclonal Antibody [Clone ID: 210H1]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    210H1
  • Gene ID85480
  • Target name
    TSLP
  • Target description
    thymic stromal lymphopoietin
  • Target synonyms
    thymic stromal lymphopoietin
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ969D9
  • Protein Name
    Thymic stromal lymphopoietin
  • Scientific Description
    TSLP mouse monoclonal antibody, clone 210H1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TSLP using anti-TSLP antibody (A01096). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSLP antigen affinity purified polyclonal antibody (Catalog # A01096) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSLP at approximately 18KD. The expected band size for TSLP is at 18KD.
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