Western blot of Ubiquitin. Anti-Ubiquitin antibody (GTX14372), generated by immunization with Ubiquitin coupled to Rabbit IgG, was tested by western blot against total cell extract from yeast. Dilution of the antibody between 1:200 and 1:1,000 showed strong reactivity with Ubiquitinated proteins. In this blot the antibody was used at a 1:500 dilution incubated overnight at 4o C in 5% non-fat dry milk in TBST. Detection occurred using a 1:2,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
Western blot of Ubiquitin. Anti-Ubiquitin antibody (GTX14372), generated by immunization with Ubiquitin coupled to Rabbit IgG, was tested by western blot against total cell extract from yeast. Dilution of the antibody between 1:200 and 1:1,000 showed strong reactivity with Ubiquitinated proteins. In this blot the antibody was used at a 1:500 dilution incubated overnight at 4o C in 5% non-fat dry milk in TBST. Detection occurred using a 1:2,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
Ubiquitin antibody
GTX14372
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityOther Species
Overview
- SupplierGeneTex
- Product NameUbiquitin antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:200-1:1000. ELISA: 1:1000-1:5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration5 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- Scientific DescriptionUbiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has a normal role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimers disease, Lewy bodies seen in Parkinsons disease, and Pick bodies found in Picks disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203