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ProductsBack/UMOD / Uromodulin Antibody (aa352-380, PE)

UMOD / Uromodulin Antibody (aa352-380, PE)

LS-C273039
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetUMOD
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    UMOD / Uromodulin Antibody (aa352-380, PE)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC, WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    RPE
  • Estimated Purity
    ...
  • Gene ID7369
  • Target name
    UMOD
  • Target description
    uromodulin
  • Target synonyms
    ADMCKD2; ADTKD1; FJHN; HNFJ; HNFJ1; MCKD2; Tamm-Horsfall urinary glycoprotein; THGP; THP; uromodulin; uromucoid
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UMOD using anti-UMOD antibody (A01303-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse kidney tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey kidney tissue lysates, Lane 4: rat PC-12 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UMOD antigen affinity purified polyclonal antibody (Catalog # A01303-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UMOD at approximately 110KD. The expected band size for UMOD is at 110KD.
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