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USP10 inhibition by Ub-Aldehyde.
USP10 inhibition by Ub-Aldehyde.
USP10 inhibition by Ub-Aldehyde.

USP10 Inhibitor Screening Assay Kit

Research Use Only
79094
BPS Bioscience
Product group Assays
Price on request
Packing Size
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Overview

  • Supplier
    BPS Bioscience
  • Product Name
    USP10 Inhibitor Screening Assay Kit
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • Scientific Description
    The USP10 Inhibitor Screening Assay Kit is a fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme USP10 (Ubiquitin Specific Peptidase 10) for screening and profiling applications. The kit comes in a convenient 96-well format and contains enough purified recombinant human USP10 protein, Ubiquitinated-AMC substrate, and assay buffer for 100 reactions. To determine the effect of an inhibitor on USP10 activity the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU) and Machado-Josephin Domain (MJD) proteases. Ubiquitin-AMC (Ub-AMC) is a fluorogenic substrate for ubiquitin hydrolases, based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). In the conjugated form the energy emitted from the fluorochrome AMC is quenched. Upon release from Ub, AMC emissions are no longer quenched and fluorescence with lambdaexcitation/lambdaemission maxima of 350/460 nm is emitted. The increase in fluorescence is proportional to the DUB activity.
  • Storage Instruction
    See manual
  • UNSPSC
    41116133

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