Bio-Connect
USP14 activity is inhibited by Ub-Aldehyde.
USP14 activity is inhibited by Ub-Aldehyde.
USP14 activity is inhibited by Ub-Aldehyde.

USP14 Inhibitor Screening Assay Kit

Research Use Only
78865
BPS Bioscience
Product group Assays
Price on request
Packing Size
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    BPS Bioscience
  • Product Name
    USP14 Inhibitor Screening Assay Kit
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • Scientific Description
    The Ubiquitin specific peptidase 14 (USP14) Inhibitor Screening Assay Kit is a 96-well fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme USP14 for screening and profiling applications. The kit contains enough purified USP14 protein, Ubiquitinated-AMC substrate, and assay buffer for 100 reactions. To determine the effect of an inhibitor on USP14 activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases. Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). In conjugated form, the energy emitted from the fluorochrome AMC is quenched. Upon proteolysis, AMC is no longer quenched and emits fluorescence with lambdaexcitation/lambdaemission maxima of 350/460 nm. The increase in fluorescence is proportional to the DUB activity.
  • Storage Instruction
    See manual
  • UNSPSC
    41116133

Related products

USP14, FLAG-tag Recombinant
Product group Molecular Biology
BPS Bioscience
  • SizePrice