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USP21 Antibody (aa406-436, Azide-free, HRP)

LS-C221083
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetUSP21
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    USP21 Antibody (aa406-436, Azide-free, HRP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC, WB The applications listed have been tested for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID27005
  • Target name
    USP21
  • Target description
    ubiquitin specific peptidase 21
  • Target synonyms
    deubiquitinating enzyme 21; NEDD8-specific protease; ubiquitin carboxyl-terminal hydrolase 21; ubiquitin specific protease 21; ubiquitin thioesterase 21; ubiquitin thiolesterase 21; ubiquitin-specific processing protease 21; ubiquitin-specific protease 16; USP16; USP23
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of USP21 using anti-USP21 antibody (A06639-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP21 antigen affinity purified polyclonal antibody (Catalog # A06639-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP21 at approximately 63KD. The expected band size for USP21 is at 63KD.
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