1:000 (1microg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2)
1:000 (1microg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2)
V5 tag antibody [E10 / V4RR]
GTX82563
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityOther Species
Overview
- SupplierGeneTex
- Product NameV5 tag antibody [E10 / V4RR]
- Delivery Days Customer9
- Antibody SpecificityRecognizes V5-tagged fusion proteins
- Application Supplier NoteRecommended Starting Dilutions:WB (with ECL): Use at a dilution of 1:1000-3000 Immunostaining: Use at a dilution of 1:500 - 1:2000For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDE10 / V4RR
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- Scientific DescriptionThe V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203