![ICC/IF analysis of PFA-fixed C6 cells using GTX00942 Vimentin antibody [GT1180]. Orange : Primary antibody Blue : DAPI Dilution : 1:100 ICC/IF analysis of PFA-fixed C6 cells using GTX00942 Vimentin antibody [GT1180]. Orange : Primary antibody Blue : DAPI Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20200327_ICC-IF_4_w_23053121_726.webp)
ICC/IF analysis of PFA-fixed C6 cells using GTX00942 Vimentin antibody [GT1180]. Orange : Primary antibody Blue : DAPI Dilution : 1:100
Vimentin antibody [GT1180]
GTX00942
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVIM
Overview
- SupplierGeneTex
- Product NameVimentin antibody [GT1180]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. IP: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1180
- ConjugateUnconjugated
- Gene ID7431
- Target nameVIM
- Target descriptionvimentin
- Target synonymsvimentin, epididymis secretory sperm binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP08670
- Protein NameVimentin
- Scientific DescriptionThis gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Zhang MH, Liu J. Cleavage stimulation factor 2 promotes malignant progression of liver hepatocellular carcinoma by activating phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of rapamycin pathway. Bioengineered. 2022,13(4):10047-10060. doi: 10.1080/21655979.2022.2063100Read this paper
![ICC/IF analysis of PFA-fixed HeLa cells using GTX00942 Vimentin antibody [GT1180]. Orange : Primary antibody Blue : DAPI Dilution : 1:100 ICC/IF analysis of PFA-fixed HeLa cells using GTX00942 Vimentin antibody [GT1180]. Orange : Primary antibody Blue : DAPI Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20200327_ICC-IF_5_w_23053121_500.webp)
![ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00942 Vimentin antibody [GT1180]. Orange : Primary antibody Blue : DAPI Dilution : 1:100 ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00942 Vimentin antibody [GT1180]. Orange : Primary antibody Blue : DAPI Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20200327_ICC-IF_6_w_23053121_687.webp)
![IHC-P analysis of Rat spleen tissue section using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:100 IHC-P analysis of Rat spleen tissue section using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20200327_IHC-P_1_w_23053121_406.webp)
![IHC-P analysis of Human colon carcinoma tissue section using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:100 IHC-P analysis of Human colon carcinoma tissue section using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20200327_IHC-P_2_w_23053121_228.webp)
![IHC-P analysis of Mouse kidney tissue section using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:100 IHC-P analysis of Mouse kidney tissue section using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20200327_IHC-P_3_w_23053121_381.webp)
![WB analysis of various samples using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:1000 Loading : 25microg WB analysis of various samples using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20200327_WB_1_w_23053121_319.webp)
![WB analysis of wild-type (WT) and Vimentin knockout (KO) 293T cell extracts using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:1000 Laoding : 25microg WB analysis of wild-type (WT) and Vimentin knockout (KO) 293T cell extracts using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:1000 Laoding : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20200327_WB_2_w_23053121_551.webp)
![IP analysis of Jurkat cell lysate using GTX00942 Vimentin antibody [GT1180]. Antibody amount : 3microg / 300microg lysate Dilution : 1:1000 IP analysis of Jurkat cell lysate using GTX00942 Vimentin antibody [GT1180]. Antibody amount : 3microg / 300microg lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20210323_IP_13_w_23053121_713.webp)
![Various whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT1180] (GTX00942) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT1180] (GTX00942) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_40000000086_20200306_WB_w_23053121_600.webp)





![IHC-P analysis of human melanoma tissue using GTX80814 Vimentin antibody [J144]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX80814/GTX80814_1419_IHC-P_w_23061322_923.webp)