ICC/IF analysis of PFA-fixed C6 cells using GTX00942 Vimentin antibody [GT1180]. Orange : Primary antibody Blue : DAPI Dilution : 1:100
![ICC/IF analysis of PFA-fixed HeLa cells using GTX00942 Vimentin antibody [GT1180]. Orange : Primary antibody Blue : DAPI Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20200327_ICC-IF_5_w_23053121_500.webp "ICC/IF analysis of PFA-fixed HeLa cells using GTX00942 Vimentin antibody [GT1180]. Orange : Primary antibody Blue : DAPI Dilution : 1:100")
![ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00942 Vimentin antibody [GT1180]. Orange : Primary antibody Blue : DAPI Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20200327_ICC-IF_6_w_23053121_687.webp "ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00942 Vimentin antibody [GT1180]. Orange : Primary antibody Blue : DAPI Dilution : 1:100")
![IHC-P analysis of Rat spleen tissue section using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20200327_IHC-P_1_w_23053121_406.webp "IHC-P analysis of Rat spleen tissue section using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:100")
![IHC-P analysis of Human colon carcinoma tissue section using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20200327_IHC-P_2_w_23053121_228.webp "IHC-P analysis of Human colon carcinoma tissue section using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:100")
![IHC-P analysis of Mouse kidney tissue section using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20200327_IHC-P_3_w_23053121_381.webp "IHC-P analysis of Mouse kidney tissue section using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:100")
![WB analysis of various samples using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20200327_WB_1_w_23053121_319.webp "WB analysis of various samples using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:1000 Loading : 25microg")
![WB analysis of wild-type (WT) and Vimentin knockout (KO) 293T cell extracts using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:1000 Laoding : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20200327_WB_2_w_23053121_551.webp "WB analysis of wild-type (WT) and Vimentin knockout (KO) 293T cell extracts using GTX00942 Vimentin antibody [GT1180]. Dilution : 1:1000 Laoding : 25microg")
![IP analysis of Jurkat cell lysate using GTX00942 Vimentin antibody [GT1180]. Antibody amount : 3microg / 300microg lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_20210323_IP_13_w_23053121_713.webp "IP analysis of Jurkat cell lysate using GTX00942 Vimentin antibody [GT1180]. Antibody amount : 3microg / 300microg lysate Dilution : 1:1000")
![Various whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT1180] (GTX00942) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00942/GTX00942_40000000086_20200306_WB_w_23053121_600.webp "Various whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT1180] (GTX00942) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
ICC/IF analysis of PFA-fixed C6 cells using GTX00942 Vimentin antibody [GT1180]. Orange : Primary antibody Blue : DAPI Dilution : 1:100
Vimentin antibody [GT1180] - Recombinant antibody, KO/KD, Orthogonal Validated
GTX00942
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVIM
Overview
- SupplierGeneTex
- Product NameVimentin antibody [GT1180] - Recombinant antibody, KO/KD, Orthogonal Validated
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1180
- ConjugateUnconjugated
- Gene ID7431
- Target nameVIM
- Target descriptionvimentin
- Target synonymsepididymis secretory sperm binding protein; vimentin
- HostRabbit
- IsotypeIgG
- Scientific DescriptionThis gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
- ReactivityHuman, Mouse, Rat
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
- Cleavage stimulation factor 2 promotes malignant progression of liver hepatocellular carcinoma by activating phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin pathway. Zhang MH et al., 2022 Apr, BioengineeredRead more