Western blot analysis of extracts of HeLa cells, using Phospho-Vimentin-S83 pAb (TA383239) at 1:2000 dilution or Vimentin antibody (A2584).HeLa cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours or treated by Paclitaxel (100 nM/mL) at 37°C for 20 hours. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 1s.
Western blot analysis of extracts of HeLa cells, using Phospho-Vimentin-S83 pAb (TA383239) at 1:2000 dilution or Vimentin antibody (A2584).HeLa cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours or treated by Paclitaxel (100 nM/mL) at 37°C for 20 hours. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 1s.
Vimentin (VIM) Rabbit Polyclonal Antibody
TA383239
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetVIM
Overview
- SupplierOriGene
- Product NameVimentin (VIM) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID7431
- Target nameVIM
- Target descriptionvimentin
- Target synonymsepididymis secretory sperm binding protein; vimentin
- HostRabbit
- IsotypeIgG
- Scientific DescriptionPhospho-Vimentin-S83 Rabbit polyclonal Antibody
- ReactivityHuman, Rat
- UNSPSC12352203