Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Vimentin-S39 antibody (TA383241) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37°C for 20 hours.NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 1s.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Vimentin-S39 antibody (TA383241) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37°C for 20 hours.NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 1s.
Vimentin (VIM) Rabbit Polyclonal Antibody
TA383241
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVIM
Overview
- SupplierOriGene
- Product NameVimentin (VIM) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID7431
- Target nameVIM
- Target descriptionvimentin
- Target synonymsepididymis secretory sperm binding protein; vimentin
- HostRabbit
- IsotypeIgG
- Scientific DescriptionPhospho-Vimentin-S39 Rabbit polyclonal Antibody
- ReactivityHuman, Mouse, Rat
- UNSPSC12352203