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ProductsBack/Vinculin antibody [N1N3]

Vinculin antibody [N1N3]

GTX113294
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVCL
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Overview

  • Supplier
    GeneTex
  • Product Name
    Vinculin antibody [N1N3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.67 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7414
  • Target name
    VCL
  • Target description
    vinculin
  • Target synonyms
    CMD1W, CMH15, HEL114, MV, MVCL, VINC, vinculin, epididymis luminal protein 114, epididymis secretory sperm binding protein, meta-vinculin, metavinculin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18206
  • Protein Name
    Vinculin
  • Scientific Description
    Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Miglietta S, Girolimetti G, Marchio L, et al. MicroRNA and Metabolic Profiling of a Primary Ovarian Neuroendocrine Carcinoma Pulmonary-Type Reveals a High Degree of Similarity with Small Cell Lung Cancer. Noncoding RNA. 2022,8(5). doi: 10.3390/ncrna8050064
    Read this paper
  • D'Angelo L, Astro E, De Luise M, et al. NDUFS3 depletion permits complex I maturation and reveals TMEM126A/OPA7 as an assembly factor binding the ND4-module intermediate. Cell Rep. 2021,35(3):109002. doi: 10.1016/j.celrep.2021.109002
    Read this paper
  • Boschetti E, D'Angelo R, Tardio ML, et al. Evidence of enteric angiopathy and neuromuscular hypoxia in patients with mitochondrial neurogastrointestinal encephalomyopathy. Am J Physiol Gastrointest Liver Physiol. 2021,320(5):G768-G779. doi: 10.1152/ajpgi.00047.2021
    Read this paper
  • Inder S, Bates M, Ni Labhrai N, et al. Multiplex profiling identifies clinically relevant signalling proteins in an isogenic prostate cancer model of radioresistance. Sci Rep. 2019,9(1):17325. doi: 10.1038/s41598-019-53799-7
    Read this paper
  • Boschetti E, Accarino A, Malagelada C, et al. Gut epithelial and vascular barrier abnormalities in patients with chronic intestinal pseudo-obstruction. Neurogastroenterol Motil. 2019,31(8):e13652. doi: 10.1111/nmo.13652
    Read this paper
  • Haug MF, Berger M, Gesemann M, et al. Differential expression of PKCα and -β in the zebrafish retina. Histochem Cell Biol. 2019,151(6):521-530. doi: 10.1007/s00418-018-1764-8
    Read this paper
  • Ko CC, Chen YJ, Chen CT, et al. Chemical proteomics identifies heterogeneous nuclear ribonucleoprotein (hnRNP) A1 as the molecular target of quercetin in its anti-cancer effects in PC-3 cells. J Biol Chem. 2014,289(32):22078-89. doi: 10.1074/jbc.M114.553248
    Read this paper
  • Liou YR, Torng W, Kao YC, et al. Substrate stiffness regulates filopodial activities in lung cancer cells. PLoS One. 2014,9(2):e89767. doi: 10.1371/journal.pone.0089767
    Read this paper

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Figure 1. Western blot analysis of VCL using anti-VCL antibody (A01207-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human SK-OV-3 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: human K562 whole cell lysates, Lane 8: human SGC-7901 whole cell lysates, Lane 9: rat heart tissue lysates, Lane 10: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCL antigen affinity purified polyclonal antibody (Catalog # A01207-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCL at approximately 124KD. The expected band size for VCL is at 124KD.
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