Bio-Connect

VPS33A antibody [C1C3]

GTX119416
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetVPS33A
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Overview

  • Supplier
    GeneTex
  • Product Name
    VPS33A antibody [C1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID65082
  • Target name
    VPS33A
  • Target description
    VPS33A core subunit of CORVET and HOPS complexes
  • Target synonyms
    MPSPS, vacuolar protein sorting-associated protein 33A, VPS33A, CORVET/HOPS core subunit, vacuolar protein sorting 33 homolog A, vacuolar protein sorting 33A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96AX1
  • Protein Name
    Vacuolar protein sorting-associated protein 33A
  • Scientific Description
    Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and it encodes a protein similar to the yeast class C Vps33 protein. The mammalian class C VPS proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Miao G, Zhao H, Li Y, et al. ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation. Dev Cell. 2021,56(4):427-442.e5. doi: 10.1016/j.devcel.2020.12.010
    Read this paper
  • Uematsu M, Nishimura T, Sakamaki Y, et al. Accumulation of undegraded autophagosomes by expression of dominant-negative STX17 (syntaxin 17) mutants. Autophagy. 2017,13(8):1452-1464. doi: 10.1080/15548627.2017.1327940
    Read this paper
  • Hubert V, Peschel A, Langer B, et al. LAMP-2 is required for incorporating syntaxin-17 into autophagosomes and for their fusion with lysosomes. Biol Open. 2016,5(10):1516-1529. doi: 10.1242/bio.018648
    Read this paper
  • van der Kant R, Jonker CT, Wijdeven RH, et al. Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity. J Biol Chem. 2015,290(51):30280-90. doi: 10.1074/jbc.M115.688440
    Read this paper
  • Jiang P, Nishimura T, Sakamaki Y, et al. The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol Biol Cell. 2014,25(8):1327-37. doi: 10.1091/mbc.E13-08-0447
    Read this paper
  • van der Kant R, Fish A, Janssen L, et al. Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L. J Cell Sci. 2013,126(Pt 15):3462-74. doi: 10.1242/jcs.129270
    Read this paper