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ProductsBack/VTN / Vitronectin Antibody (clone BV1)

VTN / Vitronectin Antibody (clone BV1)

LS-C139970
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot
Product group Antibodies
TargetVTN
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    VTN / Vitronectin Antibody (clone BV1)
  • Delivery Days Customer
    23
  • Application Supplier Note
    For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • Applications Supplier
    Flo, IF, IP, WB For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BV1
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7448
  • Target name
    VTN
  • Target description
    vitronectin
  • Target synonyms
    complement S-protein; epibolin; serum spreading factor; somatomedin B; V75; vitronectin; VN; VNT
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Vitronectin using anti-Vitronectin antibody (PB9458). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Lung Tissue Lysate at 50ug, Lane 3: HEPG2 Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug, Lane 5: HEPA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vitronectin antigen affinity purified polyclonal antibody (Catalog # PB9458) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vitronectin at approximately 54 kDa. The expected band size for Vitronectin is at 54 kDa.
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