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ProductsBack/WIF1 Antibody (clone D50F9)

WIF1 Antibody (clone D50F9)

LS-C184164
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetWIF1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    WIF1 Antibody (clone D50F9)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    D50F9
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11197
  • Target name
    WIF1
  • Target description
    WNT inhibitory factor 1
  • Target synonyms
    WIF-1; wnt inhibitory factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WIF1 using anti-WIF1 antibody (M03247-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WIF1 antigen affinity purified monoclonal antibody (Catalog # M03247-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WIF1 at approximately 42 kDa. The expected band size for WIF1 is at 42 kDa.
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Formalin-fixed and paraffin embedded rat hippocampus labeled with Anti WIF1 Polyclonal Antibody, Unconjugated (bs-3917R) followed by conjugation to the secondary antibody and DAB staining
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