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ProductsBack/X-Gal [7240-90-6]
Chemical Structure
Chemical Structure
Chemical Structure

X-Gal [7240-90-6]

Research Use Only
AG-CC1-0003
AdipoGen Life Sciences
CAS Number7240-90-6
DownloadMSDS
Product group Chemicals
Estimated Purity>99%
Molecular Weight408.6
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    X-Gal [7240-90-6]
  • Delivery Days Customer
    10
  • CAS Number
    7240-90-6
  • Certification
    Research Use Only
  • Estimated Purity
    >99%
  • Molecular Formula
    C14H15BrClNO6
  • Molecular Weight
    408.6
  • Scientific Description
    Chemical. CAS: 7240-90-6. Formula: C14H15BrClNO6. MW: 408.6. Molecular biology reagent. Chromogenic substrate for beta-galactosidase. Used in gene cloning together with IPTG (Prod. No. AG-CC1-0002) to indicate whether a cell expresses the beta-galactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue/white screening. Distinguishes between recombinant and non-recombinant plasmids carrying the beta-galactosidase gene. Used in two-hybrid analysis. X-Gal is used to determine E. coli and coliform content in drinking water samples. - Molecular biology reagent. Chromogenic substrate for beta-galactosidase. Used in gene cloning together with IPTG (Prod. No. AG-CC1-0002) to indicate whether a cell expresses the beta-galactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue/white screening. Distinguishes between recombinant and non-recombinant plasmids carrying the beta-galactosidase gene. Used in two-hybrid analysis. X-Gal is used to determine E. coli and coliform content in drinking water samples.
  • SMILES
    OCC1O[C@@H](OC2=CNC3=C2C(Cl)=C(Br)C=C3)C(O)[C@H](O)[C@H]1O
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352200

References

  • Chemiluminescent assay of various enzymes using indoxyl derivatives as substrate and its applications to enzyme immunoassay and DNA probe assay: H. Arakawa, et al.; Anal. Biochem. 199, 238 (1991)
  • In situ histochemical detection of beta-galactosidase activity in lung: assessment of X-Gal reagent in distinguishing lacZ gene expression and endogenous beta-galactosidase activity: D.J. Weiss, et al.; Hum. Gene Ther. 8, 1545 (1997)
  • A novel colorimetric yeast bioassay for detecting trichothecene mycotoxins: K.H. Engler, et al.; J. Microbiol. Methods 35, 207 (1999)
  • Molecular Cloning: A Laboratory Manual 3rd Edition: J. Sambrook & D. Russell; Cold Spring Harbor Laboratory Press (2001)