XL 999

MedChem Express

XL 999 [705946-27-6]

10

705946-27-6

99.01

C26H28FN5O

445.53

Kinase Assay: [1] Kinase Inhibition Assays Kinase activities of KDR and PDGFRalpha are measured as the percent of ATP consumed following the kinase reaction using luciferaseluciferin-coupled chemiluminescence. Kinase reactions are initiated by combining test compound (XL 999), ATP, kinases and substrates in a 20 mL volume using 384-well microtiter plates. For KDR, the final reaction mixture contained 3 mM ATP, 1.6 mM poly(Glu, Tyr) 4:1 and 1.5 nM KDR of residues D807-V1356 with an N-terminal GST tag. For PDGFRalpha, the final reaction mixture contained 2 mM ATP, 10 mM MBP and 14 nM PDGFRalpha of residues Q551-L1089 with an N-terminal GST tag. The reaction mixture is incubated at room temperature for 4 h (KDR) or 2 h PDGFRalpha before a 20 mL aliquot of Kinase Glo is added and luminescence signal is measured using a Victor2 plate reader. Total ATP consumption is limited below 50% [1] .

O=C1NC2=C(C=C(NC3CCN(CC)CC3)C=C2)/C1=C(C4=CC=CC(F)=C4)/C5=NC=C(C)N5

-20°C,2°C to 8°C

12352200