XRCC6/XRCC5 Antibody

Cusabio

XRCC6/XRCC5 Antibody

20

ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

Polyclonal

Unconjugated

Rabbit

IgG

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3-5 direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5-deoxyribose-5-phosphate lyase (5-dRP lyase), by catalyzing the beta-elimination of the 5 deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5-dRP lyase activity allows to clean the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. Shankar Mitra, AAPG Bulletin, Oct 2006; 90: 1565 - 1584. Ward Giffin, Mol. Cell. Biol., Jun 1999; 19: 4065 - 4078. Huichen Wang, Nucleic Acids Res., Sep 2003; 31: 5377 - 5388. Boris Kysela, J. Biol. Chem., Jun 2003; 278: 22466 - 22474.

Human, Mouse, Rat

-20°C or -80°C

12352203