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XRN2 Antibody

CSB-PA875525XA01DOA
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityPlant
TargetXRN2
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Overview

  • Supplier
    Cusabio
  • Product Name
    XRN2 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID834261
  • Target name
    XRN2
  • Target description
    exoribonuclease 2
  • Target synonyms
    AT5G42540, AtXRN2, EXORIBONUCLEASE 2, K16E1.2, K16E1_2, exoribonuclease 2, exoribonuclease 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9FQ02
  • Protein Name
    5'-3' exoribonuclease 2
  • Reactivity
    Plant
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of XRN2 using anti-XRN2 antibody (A02797-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human COLO-320 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRN2 antigen affinity purified polyclonal antibody (Catalog # A02797-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRN2 at approximately 109KD. The expected band size for XRN2 is at 109KD.
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