Yatalase, Fungal Cell Lytic Enzymes

Cosmo Bio USA

Yatalase, Fungal Cell Lytic Enzymes

16

Research Use Only

Yatalase is used to lyse cell walls of filamentous fungi. The product is prepared from culture supernatants of Corynebacterium sp. OZ-21, and consists mainly of chitinase, chitobiase and beta-1,3-glucanase. Features Has excellent thermostability and can be stored at room temperature. Efficiently digests native chitin. Has the revitalization of Chitinase, Chitobiase, Chitosanase, and beta-1,3-Glucanarse Can be used alone to prepare protoplasts from filamentous fungi. Source Corynebacterium sp. OZ-21 Form : Lyophilized powder(containing lactose) Storage Lyophilized preparation is stable for at least 1 year at 4°C. Maintain at -20°C for long term strage. Avoid freeze/thaw cycles. Specific activities Chitinase activity : Approximately 50 units/g powder Chitobiase activity : Approximately 500 units/g powder Lytic activity against cell walls : Approximately 10,000 units/g powder Properties Optimum pH : pH 5-8 Optimum temperature for enzyme activity : 30-50°C UNIT DEFINITION Chitinase activity: One unit of chitinase activity is determined as the amount required to release 1umol of N-acetylglucosamine from chitin in 1 minute. Chitobiase activity: One unit of chitobiase activity is defined as the amount of enzyme required to release 1umol of p-nitrophenol from p-nitrophenyl-N-acetyl-beta-D-glucosaminide in 1 minute. Lytic activity: One unit of enzyme activity is defined as the amount required to cause a 1% decrease in absorbance in 1 hour.

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