Yeast Poly(A) Polymerase, recombinant, E. coli overexpressing Saccharomyces cerevisiae Poly(A) Polymerase

Jena Bioscience

Yeast Poly(A) Polymerase, recombinant, E. coli overexpressing Saccharomyces cerevisiae Poly(A) Polymerase

9

Research Use Only

600 unit/ul

Yeast Poly(A) Polymerase catalyzes the transfer of AMP to 3-hydroxyl ends of RNA molecules. The reaction is template-independent, requires ATP as substrate and Mg2+ or Mn2+ as cofactor. It works more efficiently than E. coli Poly(A) polymerase in some poly(A) tailing and RNA labeling reactions (e.g. shorter incubation time, broader acceptance of RNA template size). Polyadenylation increases (m)RNA stability and therefore translation efficiency in transfection and microinjection experiments in eukaryotic cells. For information on (m)RNA polyadenylation using in vitro transcription reaction mixes as template, refer to the Poly(A) Tailing Enzyme Testkit (#RNT-004).

-20°C

12352204