Non-infected (–) and infected (+) C6/36 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Zika virus NS5 protein antibody (GTX133327) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-infected (–) and infected (+) C6/36 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Zika virus NS5 protein antibody (GTX133327) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Zika virus NS5 protein antibody
GTX133327
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameZika virus NS5 protein antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:10000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.86 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Schutt WR, Conde JN, Mladinich MC, et al. ZIKV induction of tristetraprolin in endothelial and Sertoli cells post-transcriptionally inhibits IFNβ/λ expression and promotes ZIKV persistence. mBio. 2023,14(5):e0174223. doi: 10.1128/mbio.01742-23Read this paper
- Lu AY, Gustin A, Newhouse D, et al. Viral Protein Accumulation of Zika Virus Variants Links with Regulation of Innate Immunity for Differential Control of Viral Replication, Spread, and Response to Interferon. J Virol. 2023,97(5):e0198222. doi: 10.1128/jvi.01982-22Read this paper
- Zhou R, Li Q, Yang B, et al. Repurposing of the antihistamine mebhydrolin napadisylate for treatment of Zika virus infection. Bioorg Chem. 2022,128:106024. doi: 10.1016/j.bioorg.2022.106024Read this paper
- Riva L, Goellner S, Biering SB, et al. The Compound SBI-0090799 Inhibits Zika Virus Infection by Blocking De Novo Formation of the Membranous Replication Compartment. J Virol. 2021,95(22):e0099621. doi: 10.1128/JVI.00996-21Read this paper
- Yang D, Chu H, Lu G, et al. STAT2-dependent restriction of Zika virus by human macrophages but not dendritic cells. Emerg Microbes Infect. 2021,10(1):1024-1037. doi: 10.1080/22221751.2021.1929503Read this paper
- Goellner S, Cerikan B, Cortese M, et al. Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles. STAR Protoc. 2020,1(3):100173. doi: 10.1016/j.xpro.2020.100173Read this paper
- Conde JN, Schutt WR, Mladinich M, et al. NS5 Sumoylation Directs Nuclear Responses That Permit Zika Virus To Persistently Infect Human Brain Microvascular Endothelial Cells. J Virol. 2020,94(19). doi: 10.1128/JVI.01086-20Read this paper
- Cerikan B, Goellner S, Neufeldt CJ, et al. A Non-Replicative Role of the 3' Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation. Cell Rep. 2020,32(1):107859. doi: 10.1016/j.celrep.2020.107859Read this paper