10-HETE(10-Hydroxyeicosatetraenoic Acid) ELISA Kit

Biorbyt

10-HETE(10-Hydroxyeicosatetraenoic Acid) ELISA Kit

10

standard: 800 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 10-HETE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 10-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 10-HETE in the samples is then determined by comparing the OD of the samples to the standard curve

12.5-800 ng/mL

3.77 ng/mL

Research Use Only

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 10-HETE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 10-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 10-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.

All Species

41116158