14-3-3 theta antibody [N1C3-2] detects 14-3-3 theta protein at cytoplasm and nucleus on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. 14-3-3 theta antibody [N1C3-2] (GTX112026) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 theta antibody [N1C3-2] (GTX112026) diluted at 1:1000 and competitor's antibody (sc-732) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112026/GTX112026_40073_20170804_WB_competitor_watermark_w_23060500_844.webp "Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 theta antibody [N1C3-2] (GTX112026) diluted at 1:1000 and competitor's antibody (sc-732) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 theta antibody [N1C3-2] (GTX112026) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112026/GTX112026_40073_20160929_WB_w_23060500_324.webp "Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 theta antibody [N1C3-2] (GTX112026) diluted at 1:1000.")
14-3-3 theta antibody [N1C3-2] detects 14-3-3 theta protein at cytoplasm and nucleus on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. 14-3-3 theta antibody [N1C3-2] (GTX112026) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
14-3-3 theta antibody [N1C3-2]
GTX112026
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetYWHAQ
Overview
- SupplierGeneTex
- Product Name14-3-3 theta antibody [N1C3-2]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:20000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.98 mg/ml
- ConjugateUnconjugated
- Gene ID10971
- Target nameYWHAQ
- Target descriptiontyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
- Target synonyms14-3-3, 1C5, HS1, 14-3-3 protein theta, 14-3-3 protein T-cell, 14-3-3 protein tau, 14-3-3 theta, protein, theta, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
- HostRabbit
- IsotypeIgG
- Protein IDP27348
- Protein Name14-3-3 protein theta
- Scientific DescriptionThis gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5 UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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