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14-3-3 zeta antibody detects 14-3-3 zeta protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. 14-3-3 zeta antibody (GTX101075) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
14-3-3 zeta antibody detects 14-3-3 zeta protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. 14-3-3 zeta antibody (GTX101075) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
14-3-3 zeta antibody detects 14-3-3 zeta protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. 14-3-3 zeta antibody (GTX101075) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

14-3-3 zeta antibody

GTX101075
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetYWHAZ
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Overview

  • Supplier
    GeneTex
  • Product Name
    14-3-3 zeta antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7534
  • Target name
    YWHAZ
  • Target description
    tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
  • Target synonyms
    14-3-3-zeta, HEL-S-3, HEL-S-93, HEL4, KCIP-1, POPCHAS, YWHAD, 14-3-3 protein zeta/delta, 14-3-3 delta, 14-3-3 protein/cytosolic phospholipase A2, 14-3-3 zeta, epididymis luminal protein 4, epididymis secretory protein Li 3, epididymis secretory protein Li 93, phospholipase A2, protein kinase C inhibitor protein-1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide, tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP63104
  • Protein Name
    14-3-3 protein zeta/delta
  • Scientific Description
    This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5 UTR but that encode the same protein have been identified for this gene. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Ferré A, Chauvigné F, Gozdowska M, et al. Neurohypophysial and paracrine vasopressinergic signaling regulates aquaporin trafficking to hydrate marine teleost oocytes. Front Endocrinol (Lausanne). 2023,14:1222724. doi: 10.3389/fendo.2023.1222724
    Read this paper
  • Liu YJ, Kuo HC, Chern Y. A system-wide mislocalization of RNA-binding proteins in motor neurons is a new feature of ALS. Neurobiol Dis. 2021,160:105531. doi: 10.1016/j.nbd.2021.105531
    Read this paper
  • Chan TY, Egbert CM, Maxson JE, et al. TNK1 is a ubiquitin-binding and 14-3-3-regulated kinase that can be targeted to block tumor growth. Nat Commun. 2021,12(1):5337. doi: 10.1038/s41467-021-25622-3
    Read this paper
  • Yoon JH, Ham IH, Kim O, et al. Gastrokine 1 protein is a potential theragnostic target for gastric cancer. Gastric Cancer. 2018,21(6):956-967. doi: 10.1007/s10120-018-0828-8
    Read this paper
  • Brandi J, Dalla Pozza E, Dando I, et al. Secretome protein signature of human pancreatic cancer stem-like cells. J Proteomics. 2016,136:1-12. doi: 10.1016/j.jprot.2016.01.017
    Read this paper
  • Young K, Tweedie E, Conley B, et al. BMP9 Crosstalk with the Hippo Pathway Regulates Endothelial Cell Matricellular and Chemokine Responses. PLoS One. 2015,10(4):e0122892. doi: 10.1371/journal.pone.0122892
    Read this paper

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Figure 1. Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (A01141-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-14-3-3 zeta/delta antigen affinity purified polyclonal antibody (Catalog # A01141-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 14-3-3 zeta/delta at approximately 28 kDa. The expected band size for 14-3-3 zeta/delta is at 28 kDa.
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