1kb Plus Opti-DNA Marker

Applied Biological Materials

1kb Plus Opti-DNA Marker

9

PCR and Proteins

Research Use Only

PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 14 bands, ranging from 250 pb - 25 kb, for molecular weight standards in agarose gel electrophoresis. The 1,000 and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 microl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size. Total DNA concentration for all bands combined is 103.2ng/microl. Number of Bands 14 DNA Range 250bp-25kb Suggested Load Mix gently by pipetting up and down a few time, then directly load 5 microl into well of gel.

-20°C,2°C to 8°C

41105300