2-Ethynyl-adenosine

Jena Bioscience

2-Ethynyl-adenosine

5

Research Use Only

≥95%

C12H13N5O4

291.26 g/mol

2-Ethynyl-labeled adenosine (2-EA) can be used to measure de novo mRNA poly(A) tail synthesis in proliferating cells. 2-EA is cell permeable and incorporates into nascent mRNA transcripts both transcriptionally by RNA polymerase I,II and III and posttranscriptionally by poly(A) polymerase instead of their natural analolg adenosine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging[1]. Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

c12c(nc(nc2n(cn1)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O)C#C)N

-20°C

12352200