More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/20S Immunoproteasome Assay Kit
% Signal to Background of Continuous Real-Time TR-FRET Parkin titration (autoubiquitination): Serial dilutions of Parkin W403A from 50nM to 3.125nM and 300nM wt Parkin were mixed with UBA1, UBE2D3 and trf-Ub (pS65) mix. Reactions were initiated wit
% Signal to Background of Continuous Real-Time TR-FRET Parkin titration (autoubiquitination): Serial dilutions of Parkin W403A from 50nM to 3.125nM and 300nM wt Parkin were mixed with UBA1, UBE2D3 and trf-Ub (pS65) mix. Reactions were initiated wit
% Signal to Background of Continuous Real-Time TR-FRET Parkin titration (autoubiquitination): Serial dilutions of Parkin W403A from 50nM to 3.125nM and 300nM wt Parkin were mixed with UBA1, UBE2D3 and trf-Ub (pS65) mix. Reactions were initiated wit

20S Immunoproteasome Assay Kit

Research Use Only
SBB-KP0037
South Bay Bio
Product group Chemicals
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    South Bay Bio
  • Product Name
    20S Immunoproteasome Assay Kit
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Scientific Description
    South Bay Bios kit is designed to test for specific activity of 20S immunoproteasome. The kit provides purified 20S immunoproteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC) and Caspase-like activity of the immunoproteasome subunits beta1i/ PSMB9 (PAL-AMC) and beta5i/PSMB8 (ANW-AMC). Additionally, the compound, ONX-0914, which can be used to inhibit specifically the subunit beta5i/LMP7 20S immunoproteasome is included. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission= 445nm; allowing for a real-time read out of 20S immunoproteasome specific activity.
  • Storage Instruction
    -80°C
  • UNSPSC
    12352200

Related products

South Bay Bio
Ubiquitin Activating Enzyme E1 [UBA1; UBE1] (human) (rec.) (untagged)SBB-CE0011
Protein IDP22314
  • SizePrice
Thioester Activity Assay: UBE2D3 (UbcH5c) forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D3.
South Bay Bio
UBE2D3 [UbcH5c ] (human) (rec.) (untagged)SBB-CE0019
Protein IDP61077
  • SizePrice
Thioester Activity Assay: UBE2L3 (UbcH7) forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2L3.
South Bay Bio
UBE2L3 [UbcH7] (human) (rec.) (untagged)SBB-CE0020
Protein IDP68036
  • SizePrice
Thioester Activity Assay: UBE2D1 (UbcH5a) forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D1.
South Bay Bio
UBE2D1 [UbcH5a] (human) (rec.) (untagged)SBB-CE0021
Protein IDP51668
  • SizePrice
Thioester Activity Assay: UBE2K forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of excess DTT. The thioester assay also shows di-ubiquitin formation with addition of ATP. This confirms the activity of
South Bay Bio
UBE2K [UbcH1, E2-25K] (human) (rec.) (His)SBB-CE0022
Protein IDP61086
  • SizePrice
Thioester Activity Assay: UBE2D2 (UbcH5b) forms a thioester with UB in an ATP dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D2.
South Bay Bio
UBE2D2 [UbcH5b] (human) (rec.) (untagged)SBB-CE0027
Protein IDP62837
  • SizePrice
Ubiquitin Activating Enzyme E1 [UBA1; UBE1] (human) (rec.) (His)
South Bay Bio
Ubiquitin Activating Enzyme E1 [UBA1; UBE1] (human) (rec.) (His)SBB-CE0058
Protein IDP22314
  • SizePrice
Michaelis-Menten Kinetics: Ubiquitin Rhodamine 110 serially diluted from 1.6 to 0.05microM was digested with 30pM UcHL3 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA, at 25°C. Initial
South Bay Bio
UcH-L3 (human) (rec.) (untagged)SBB-DE0023
Protein IDP15374
  • SizePrice
South Bay Bio
PLpro (SARS Coronavirus) (rec.) (His)SBB-DE0024
Protein IDP0C6U8
  • SizePrice
Michaelis-Menten Kinetics: NEDD8 Rhodamine 110 serially diluted from 1.6 to 0.0125microM was digested with 30pM NEDP1 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 1mM TCEP, 0.1 mg/ml BSA, at 25°C. Initial velocities a
South Bay Bio
NEDP1 [SENP8] (human) (rec.) (His)SBB-DE0025
Protein IDQ96LD8
  • SizePrice