Bio-Connect

2nd Generation Packaging System Mix

LV003
Applied Biological Materials
Product group Molecular Biology
200 ul
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Overview

  • Supplier
    Applied Biological Materials
  • Product Name
    2nd Generation Packaging System Mix
  • Delivery Days Customer
    16
  • Applications
    Cloning
  • Category Supplier
    Vector and Virus
  • Certification
    Research Use Only
  • Scientific Description
    For the production of lentiviral particles, three components are generally required: 1) a lentiviral vector containing your inserts of interest, 2) one or two packaging vectors which contain all necessary viral structure proteins, 3) an envelope vector expressing Vesicular Stomatitis Virus (VSV) glycoprotein (G). In general, lentiviral vectors with a wildtype 5 LTR need the 2nd generation packaging system because these vectors require TAT for activation. However, the 2nd generation packaging mix will also support the production of 3rd lentiviral expression vector with a chimeric 5 LTR. All the lentiviral expression vectors marketed by ABM are 3rd generation with a chimeric of RSV promoter upstream of 5-LTR.
  • Storage Instruction
    -20°C
  • UNSPSC
    41106621