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ProductsBack/3CL Protease (T21I, A173V) (SARS-CoV-2) Assay Kit
Inhibition of 3CL Protease enzyme activity by increasing concentrations of GC376 (BPS Bioscience #78013).
Inhibition of 3CL Protease enzyme activity by increasing concentrations of GC376 (BPS Bioscience #78013).
Inhibition of 3CL Protease enzyme activity by increasing concentrations of GC376 (BPS Bioscience #78013).

3CL Protease (T21I, A173V) (SARS-CoV-2) Assay Kit

Research Use Only
78837
BPS Bioscience
Product group Assays
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Overview

  • Supplier
    BPS Bioscience
  • Product Name
    3CL Protease (T21I, A173V) (SARS-CoV-2) Assay Kit
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • Scientific Description
    The 3CL Protease (T21I, A173V) (SARS-CoV-2) Assay Kit is a 96-well homogeneous fluorogenic assay designed to measure the activity of 3CL Protease mutated at T21I and A173V for screening and profiling applications, with no time-consuming washing steps. 3CL inhibitor GC376 is also included as a control. The 3CL Protease Substrate is an internally quenched 14-mer fluorogenic peptide (DABCYL-KTSAVLQSGFRKME-EDANS). When the donor (EDANS) and acceptor (DABCYL) fluorophores are in close proximity the energy emitted from EDANS is quenched by DABCYL (intact substrate). Upon proteolysis by 3CL, the peptide substrate is cleaved between the glutamine and serine residues to generate the highly fluorescent peptide fragment (SGFRKME-EDANS). The fluorescence intensity increases proportionally to the activity of 3CL. More information on the substrate, including MW and structure, can be found on our website (BPS Bioscience #79952).
  • Storage Instruction
    See manual
  • UNSPSC
    41116133