4-Azido-L-homoalanine HCl (L-AHA), (S)-2-Amino-4-azidobutanoic acid hydrochloride [942518-29-8]

Jena Bioscience

4-Azido-L-homoalanine HCl (L-AHA), (S)-2-Amino-4-azidobutanoic acid hydrochloride [942518-29-8]

5

942518-29-8

Research Use Only

>99%

C4H8N4O2 * HCl

144.13 g/mol + 36.45 g/mol

4-Azido-L-homoalanine (L-AHA) provides a non-radioactive alternative to analyze the global protein synthesis in cell culture. It is cell-permeable and randomly incorporated instead of methionine during translation[1,2,3]. The resulting azide-labeled full-length proteins can subsequently be detected via Cu(I)-catalyzed or Cu(I)-free click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin or DBCO-containing Biotin, respectively) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes or DBCO-containing fluorescent dyes, respectively) for subsequent microscopic imaging. Presolski et al.[4] and Hong et al.[5] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

OC(=O)[C@H](CCN=[N+]=[N-])N

2°C to 8°C

12352200