Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:3000 and 4E-BP1 antibody [N1C3] (GTX109162) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX133181/GTX133181_42640_20251024_WB_treatment_CIP_25103019_542.webp "Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:3000 and 4E-BP1 antibody [N1C3] (GTX109162) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![Untreated (–) and treated (+) C2C12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:1000 and 4E-BP1 antibody [N1C3] (GTX109162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX133181/GTX133181_42640_20251024_WB_M_treatment_CIP_25103019_340.webp "Untreated (–) and treated (+) C2C12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:1000 and 4E-BP1 antibody [N1C3] (GTX109162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
4E-BP1 (phospho Thr37/Thr46) antibody
GTX133181
ApplicationsWestern Blot
Product group Antibodies
ReactivityDrosophila, Human, Mouse, Rat
TargetEIF4EBP1
Overview
- SupplierGeneTex
- Product Name4E-BP1 (phospho Thr37/Thr46) antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.17 mg/ml
- ConjugateUnconjugated
- Gene ID1978
- Target nameEIF4EBP1
- Target descriptioneukaryotic translation initiation factor 4E binding protein 1
- Target synonyms4E-BP1, 4EBP1, BP-1, PHAS-I, eukaryotic translation initiation factor 4E-binding protein 1, eIF4E-binding protein 1, phosphorylated heat- and acid-stable protein regulated by insulin 1
- HostRabbit
- IsotypeIgG
- Scientific DescriptionThis gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5 end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq]
- ReactivityDrosophila, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Cardile A, Zanrè V, Campagnari R, et al. Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism. Int J Mol Sci. 2023,24(2). doi: 10.3390/ijms24021263Read this paper
Datasheet
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