5-Bromo-3-indolyl beta-D-galactopyranoside [97753-82-7]

MedChem Express

5-Bromo-3-indolyl beta-D-galactopyranoside [97753-82-7]

10

97753-82-7

Research Use Only

99.73

C14H16BrNO6

374.18

5-Bromo-3-indolyl beta-D-galactopyranoside (Bluo-Gal) is a chromogenic substrate for beta-galactosidase. 5-Bromo-3-indolyl beta-D-galactopyranoside is hydrolyzed by the enzyme to generate a 5-bromoindole intermediate, which is further oxidized to form an insoluble blue precipitate. 5-Bromo-3-indolyl beta-D-galactopyranoside can specifically recognize bacterial beta-galactosidases (such as the product of the Escherichia coli lacZ gene) and reacts at pH 7.4, making it suitable for light and electron microscopic observations. 5-Bromo-3-indolyl beta-D-galactopyranoside can be used in histochemical detection of reporter gene expression in transgenic organisms, such as the localization analysis of beta-galactosidase activity in mouse embryos or muscle tissues[1][2].

BrC1=CC=C2C(C(O[C@@H]([C@@H]([C@H]3O)O)O[C@@H]([C@@H]3O)CO)=CN2)=C1

2°C to 8°C

12162000