5-DBCO-PEG4-dUTP

Jena Bioscience

5-DBCO-PEG4-dUTP

5

Research Use Only

10-11 mM

≥95%

C42H54N5O21P3 (free acid)

1057.83 g/mol (free acid)

5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasksto introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imagingto crosslink the DNA to Azide-functionalized biomolecules e.g.proteins

c1(=O)[nH]c(=O)n(cc1/C=C/CNC(=O)CCOCCOCCOCCOCCNC(=O)CCC(=O)N1Cc2c(C#Cc3c1cccc3)cccc2)[C@H]1C[C@H](O)[C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O

-20°C

12352200