Bio-Connect

5-DBCO-PEG4-dUTP

Research Use Only
ORB532299
Biorbyt
Product group Molecular Biology
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Overview

  • Supplier
    Biorbyt
  • Product Name
    5-DBCO-PEG4-dUTP
  • Delivery Days Customer
    16
  • Application Supplier Note
    Incorporation into DNA/cDNA by PCR with Taqpolymerasein-house data. Spectroscopic Propertie: lambdamax 289 nm, epsilon 12.5 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
  • Certification
    Research Use Only
  • Concentration
    10-11 mM
  • Formulation
    Liquid
  • Scientific Description
    5-DBCO-PEG 4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The result-ing DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free click chemistry that offers the choice: to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks; to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352200