5-DBCO-PEG4-dUTP

Biorbyt

5-DBCO-PEG4-dUTP

16

Incorporation into DNA/cDNA by PCR with Taqpolymerasein-house data. Spectroscopic Propertie: lambdamax 289 nm, epsilon 12.5 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

10-11 mM

Liquid

5-DBCO-PEG 4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The result-ing DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free click chemistry that offers the choice: to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks; to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins.

-20°C

12352200