5-Ethynyl-2-deoxycytidine (5-EdC)

Jena Bioscience

5-Ethynyl-2-deoxycytidine (5-EdC)

5

Research Use Only

≥99%

C11H13N3O4

251.24 g/mol

Ethynyl-labeled deoxycytidine (5-EdC) can be used as a replacement for BrdU (5-Bromo-2-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-EdC is cell-permeabel and incorporates into replicating DNA instead of its natural analog thymidine. The resulting ethynyl-functionalized DNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (Azides of Biotin) for subsequent purification tasks or a fluorescent group (Azides of fluorescent dyes) for subsequent microscopic imaging [1]. Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

n1(cc(c(nc1=O)N)C#C)[C@H]1C[C@@H]([C@H](O1)CO)O

-20°C

12352200