5-Ethynyl-2-deoxyuridine (5-EdU) [61135-33-9]

Jena Bioscience

5-Ethynyl-2-deoxyuridine (5-EdU) [61135-33-9]

5

61135-33-9

Research Use Only

≥98%

C11H12N2O5

252.23 g/mol

5-EdU (5-Ethynyl-2-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-EdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymidine. The resulting ethynyl-functionalized DNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging [1-5]. Presolski et al.[6] and Hong et al.[7] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

n1(cc(c(=O)[nH]c1=O)C#C)[C@H]1C[C@@H]([C@H](O1)CO)O

-20°C

12352200