5-Ethynyl-uridine (5-EU) [69075-42-9]

Jena Bioscience

5-Ethynyl-uridine (5-EU) [69075-42-9]

5

69075-42-9

Research Use Only

≥99%

C11H12N2O6

268.22 g/mol

Ethynyl-labeled uridine (5-EU) can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo RNA synthesis in proliferating cells. 5-EU is cell permeable and incorporates into nascent RNA instead of its natural analog uridine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging[1]. Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

n1(cc(c(=O)[nH]c1=O)C#C)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O

-20°C

12352200