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Chemical Structure

5-FAM (high purity) [76823-03-5]

CDX-C1009
Chemodex
CAS Number76823-03-5
Product group Chemicals
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Overview

  • Supplier
    Chemodex
  • Product Name
    5-FAM (high purity)
  • Delivery Days Customer
    10
  • CAS Number
    76823-03-5
  • Scientific Description
    5-FAM is the amine-reactive purified single isomer of 5,6-carboxyfluorescein and is used for labeling peptides, proteins and nucleotides through the interaction of carboxylic acid with primary amines. It has been used to develop a variety of green fluorescent reagents and small fluorescent molecules due to its relatively high absorbance characteristics, excellent fluorescence quantum yield, and good water solubility. The probe has also found application in studies of liposome-cell, cell-cell, and liposome-liposome interactions and in related studies on lipid bilayer structures. Spectral data: lambdaex = 494nm; lambdaem = 518nm. Carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8-7.4). Carboxyfluorescein is commonly employed as a polar tracer. - Chemical. CAS: 76823-03-5. Formula: C21H12O7. MW: 376.32. Soluble in water, DMSO or DMF. 5-FAM is the amine-reactive purified single isomer of 5,6-carboxyfluorescein and is used for labeling peptides, proteins and nucleotides through the interaction of carboxylic acid with primary amines. It has been used to develop a variety of green fluorescent reagents and small fluorescent molecules due to its relatively high absorbance characteristics, excellent fluorescence quantum yield, and good water solubility. The probe has also found application in studies of liposome-cell, cell-cell, and liposome-liposome interactions and in related studies on lipid bilayer structures. Spectral data: lambdaex = 494nm; lambdaem = 518nm. Carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8-7.4). Carboxyfluorescein is commonly employed as a polar tracer.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352200

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