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5-HT-2B Antibody (OAAF06028)

OAAF06028
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHTR2B
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    5-HT-2B Antibody (OAAF06028)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID3357
  • Target name
    HTR2B
  • Target description
    5-hydroxytryptamine receptor 2B
  • Target synonyms
    5-HT(2B), 5-HT-2B, 5-HT2B, 5-hydroxytryptamine receptor 2B, 5-HT 2B receptor, 5-hydroxytryptamine (serotonin) receptor 2B, G protein-coupled, 5-hydroxytryptamine 2B receptor, serotonin receptor 2B
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of 5HT2B Receptor using anti-5HT2B Receptor antibody (PB9600). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: U87 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-5HT2B Receptor antigen affinity purified polyclonal antibody (Catalog # PB9600) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 5HT2B Receptor at approximately 54 kDa. The expected band size for 5HT2B Receptor is at 54 kDa.
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