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Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti 5-HTR2A/HTR2A Polyclonal Antibody, Unconjugated (bs-1056R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti 5-HTR2A/HTR2A Polyclonal Antibody, Unconjugated (bs-1056R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti 5-HTR2A/HTR2A Polyclonal Antibody, Unconjugated (bs-1056R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

5HT2A Receptor Polyclonal Antibody

BS-1056R
Bioss
ApplicationsWestern Blot
Product group Antibodies
TargetHTR2A
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Overview

  • Supplier
    Bioss
  • Product Name
    5HT2A Receptor Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3356
  • Target name
    HTR2A
  • Target description
    5-hydroxytryptamine receptor 2A
  • Target synonyms
    5-HT2A, HTR2, 5-hydroxytryptamine receptor 2A, 5-HT2 receptor, 5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled, serotonin 5-HT-2A receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28223
  • Protein Name
    5-hydroxytryptamine receptor 2A
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of 5HT2A Receptor using anti-5HT2A Receptor antibody (PB9599). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Testis Tissue Lysate, Lane 3: U87 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-5HT2A Receptor antigen affinity purified polyclonal antibody (Catalog # PB9599) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 5HT2A Receptor at approximately 53KD. The expected band size for 5HT2A Receptor is at 53KD.
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