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5-Nucleotidase, Mitochondrial (NT5M) Polyclonal Antibody

CAU22975
Biomatik
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetNT5M
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Overview

  • Supplier
    Biomatik
  • Product Name
    5'-Nucleotidase, Mitochondrial (NT5M) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID56953
  • Target name
    NT5M
  • Target description
    5',3'-nucleotidase, mitochondrial
  • Target synonyms
    dNT-2, dNT2, mdN, 5'(3')-deoxyribonucleotidase, mitochondrial, 5' nucleotidase, mitochondrial, 5(3)-deoxyribonucleotidase, deoxy-5'-nucleotidase 2, mitochondrial 5' nucleotidase
  • Host
    Rabbit
  • Protein IDQ9NPB1
  • Protein Name
    5'(3')-deoxyribonucleotidase, mitochondrial
  • Scientific Description
    The 5-Nucleotidase, Mitochondrial (NT5M) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NT5M using anti-NT5M antibody (A12979-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NT5M antigen affinity purified polyclonal antibody (Catalog # A12979-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NT5M at approximately 26 kDa. The expected band size for NT5M is at 26 kDa.
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