5-Propargylamino-dCTP-ATTO-647N

ORB533139
Product group Molecular Biology
Overview
- SupplierBiorbyt
- Product Name5-Propargylamino-dCTP-ATTO-647N
- Delivery Days Customer16
- Application Supplier NoteSpectroscopic Propertie: lambdaexc 646 nm, lambdaem 664 nm, epsilon 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
- CertificationResearch Use Only
- Concentration1.0-1.1 mM
- FormulationLiquid
- Scientific Description5-Propargylamino-dCTP-ATTO-647N is recommended for direct enzy-matic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-ATTO-647N/dCTP ratio for Nick Translation: 30-50% Propargylamino-dCTP-ATTO-647N/70-50% dCTP.
- Storage Instruction-20°C
- UNSPSC12352200