5-Propargylamino-dCTP-Cy3

ORB533140
Product group Molecular Biology
Overview
- SupplierBiorbyt
- Product Name5-Propargylamino-dCTP-Cy3
- Delivery Days Customer16
- Application Supplier NoteIncorporation into DNA/cDNA by: Primer Extension with Klenow fragment; PCR with Taqpolymerase in-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: lambdaexc 550 nm, lambdaem 570 nm, epsilon 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
- CertificationResearch Use Only
- Concentration1.0-1.1 mM
- FormulationLiquid
- Scientific Description5-Propargylamino-dCTP-Cy3 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-Cy3/dCTP ratio for PCR and Nick Translation: 30-50% Propargylamino-dCTP-Cy3/70-50% dCTP.
- Storage Instruction-20°C
- UNSPSC12352200