5-Propargylamino-dCTP-Cy5

Biorbyt

5-Propargylamino-dCTP-Cy5

16

Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: lambdaexc 649 nm, lambdaem 670 nm, epsilon 250.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

1.0-1.1 mM

Liquid

5-Propargylamino-dCTP-Cy5 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-Cy5/dCTP ratio for PCR and Nick Translation: 30-50% Propargylamino-dCTP-Cy5/70-50% dCTP.

-20°C

12352200