5-Propargylamino-dCTP - Solution
ORB1733845
CAS Number115899-39-3
Product group Chemicals
Estimated Purity≥ 93% (HPLC)
Molecular WeightTheoretical MW: 520.22 g/mol (free acid); Detected MW: 520.02 g/mol (free acid)
Overview
- SupplierBiorbyt
- Product Name5-Propargylamino-dCTP - Solution [115899-39-3]
- Delivery Days Customer10
- Application Supplier NoteIncorporation into DNA by PCR with Taqpolymerasein-house data. Spectroscopic Propertie: lambdamax 294 nm, epsilon 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
- CAS Number115899-39-3
- CertificationResearch Use Only
- Concentration100 mM-110 mM
- Estimated Purity≥ 93% (HPLC)
- Molecular WeightTheoretical MW: 520.22 g/mol (free acid); Detected MW: 520.02 g/mol (free acid)
- Scientific DescriptionPropargylamino-dCTP is recommended for two-step labeling of DNA e.g. by PCR. It is enzymatically incorporated into DNA as substitute for its natural counterpart dCTP. The resulting Amine-functionalized DNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice: to introduce a Biotin group (via NHS Ester of Biotin) for subse-quent purification tasks; to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging.
- Storage Instruction-20°C,RT
- UNSPSC12352200